Principle of 3 D disinfection
Steinemann Disinfection's technology is based on an automated process for the uniform disinfection of air and surfaces.
The system generates a microfine aerosol from hydrogen peroxide (H₂O₂) in a dynamic vortex whose droplets are in the micrometer range. These remain in the air long enough to inactivate airborne microorganisms before they are evenly deposited on all surfaces – even in hidden or hard-to-reach areas.
The result is a complete, reproducible and validated disinfectant effect that significantly exceeds the capabilities of manual procedures.
Microbiology and efficacy
The effectiveness of the technology is based on the oxidative effect of hydrogen peroxide. Upon contact with microorganisms, reactive oxygen species (ROS) are formed, which destroy cell membranes, proteins and nucleic acids. This reliably inactivates bacteria, viruses, fungi and spores – even highly resistant pathogens such as Clostridioides difficile or non-enveloped viruses (e.g. B. Adeno- or noroviruses).
Laboratory and practical tests show a significant reduction even under real application conditions.
Source: Andersen, BM et al. (2006): Effect of aerosolized hydrogen peroxide on spores in rooms and ambulances. Journal of Hospital Infection. PMID: 16337307
After the process is complete, hydrogen peroxide completely breaks down into water and oxygen. No chlorinated residue, no toppings and no interference with materials are produced, a major advantage over many conventional disinfectants.
Standards and tests
All CleanCube systems are validatable according to EN 17272:2020, the European standard for automated procedures for room and air disinfection.
This standard specifies how the bactericidal, sporicidal, fungicidal, virucidal and mycobactericidal efficacy of disinfection systems is tested under practical conditions.
Validation is carried out by:
Placement of biological indicators at several spatial positions, including zones that are difficult to access
Measurement and monitoring of concentration, temperature and relative humidity Complete documentation of each cycle for traceability and audibility Source: BS EN 17272:2020 – Chemical disinfectants and antiseptics. Methods of airborne room disinfection by automated process. British Standards Institution (BSI). Steinemann Disinfection thus ensures that every disinfection process is reproducible, measurable and auditable – with consistently high microbiological efficacy.
Whitepaper & Expertise on Validated Room Disinfection
Deepen your knowledge of automated aerosol disinfection, EN 17272 and the scientific basis of validated room decontamination. Our white papers provide a concise summary of the current state of studies, standards and practices.
Automated aerosol systems, science, validated room disinfection
Why room disinfection must be engineered rather than improvised – from log reduction and EN 17272 to validation with chemical and biological indicators.
Read WhitepaperWhy manual disinfection isn't enough – why cold fogging makes the difference
Evidence-based representation of the limits of manual area disinfection (only 49% correctly cleaned high-touch areas, untreated indoor air, persistent pathogens/biofilms) and how CleanCube's H₂O₂ cold fogging closes these gaps with a validated 3D process – including clinical evidence and 17 references.
Read WhitepaperUnderstanding the Relationship Between Room Volume, Target Dosage and Operating Time
Explains the technical calculation model of the CleanCube: How the operating time is deterministically derived from room volume, target dosage (ml/m³) and pump power – including formulas, calculation examples and practical tables.
Read WhitepaperHealth and environmental effects of aerosolized hydrogen peroxide in room disinfection
Health and environmental effects of aerosolized H₂O₂: exposure pathways, limit 1 ppm, effects on humans and animals, environmental compatibility and safe process management (monitoring, ventilation, organization).
Read technical noteClassification of Biological Indicators with G. stearothermophilus for Verification (EN 17272)
Compares two testing concepts for verifying H₂O₂ aerosol systems: biological indicators (spores as "worst-case" test markers) versus EN-17272 organism tests, including resistance hierarchy and log₁₀ reduction logic.
Read technical notePersonal Protective Equipment for H2O2
This Technical Note explains when and what personal protective equipment is required for safe access during H₂O₂-based room disinfection.
Read technical note (EN)Applications in practice and industry
Steinemann Disinfection's 3 D disinfection technology is used in a wide variety of environments – wherever hygiene determines safety, efficiency and trust. Our systems enable reproducible disinfection processes that are validated, documented and residue-free. It treats both the air and any surface in the room – a crucial advance over manual procedures.