Principle of 3 D disinfection
Steinemann Disinfection's technology is based on an automated process for the uniform disinfection of air and surfaces.
The system generates a microfine aerosol from hydrogen peroxide (H₂O₂) in a dynamic vortex whose droplets are in the micrometer range. These remain in the air long enough to inactivate airborne microorganisms before they are evenly deposited on all surfaces – even in hidden or hard-to-reach areas.
The result is a complete, reproducible and validated disinfectant effect that significantly exceeds the capabilities of manual procedures.
Microbiology and efficacy
The effectiveness of the technology is based on the oxidative effect of hydrogen peroxide. Upon contact with microorganisms, reactive oxygen species (ROS) are formed, which destroy cell membranes, proteins and nucleic acids. This reliably inactivates bacteria, viruses, fungi and spores – even highly resistant pathogens such as Clostridioides difficile or non-enveloped viruses (e.g. B. Adeno- or noroviruses).
Laboratory and practical tests show a significant reduction even under real application conditions.
Source: Andersen, BM et al. (2006): Effect of aerosolized hydrogen peroxide on spores in rooms and ambulances. Journal of Hospital Infection. PMID: 16337307
After the process is complete, hydrogen peroxide completely breaks down into water and oxygen. No chlorinated residue, no toppings and no interference with materials are produced, a major advantage over many conventional disinfectants.
Standards and tests
All CleanCube systems are validatable according to EN 17272:2020, the European standard for automated procedures for room and air disinfection.
This standard specifies how the bactericidal, sporicidal, fungicidal, virucidal and mycobactericidal efficacy of disinfection systems is tested under practical conditions.
Validation is carried out by:
Placement of biological indicators at several spatial positions, including zones that are difficult to access
Measurement and monitoring of concentration, temperature and relative humidity Complete documentation of each cycle for traceability and audibility Source: BS EN 17272:2020 – Chemical disinfectants and antiseptics. Methods of airborne room disinfection by automated process. British Standards Institution (BSI). Steinemann Disinfection thus ensures that every disinfection process is reproducible, measurable and auditable – with consistently high microbiological efficacy.
Whitepaper & Expertise on Validated Room Disinfection
Deepen your knowledge of automated aerosol disinfection, EN 17272 and the scientific basis of validated room decontamination. Our white papers provide a concise summary of the current state of studies, standards and practices.
Automated aerosol systems & the science of validated room disinfection
Why room disinfection must be engineered and not improvised. The white paper explains the basics – from log reduction and the concentration-time principle to aerosol behavior and droplet spectrum up to EN 17272, as well as validation with chemical and biological indicators. Ideal for HVAC planners, hygiene managers and operators who want to make disinfection measurable and reproducible.
Why manual disinfection isn't enough – why cold fogging makes the difference
Studies show that, on average, only 49% of «high-touch» surfaces are cleaned correctly, the room air remains untreated, and spores, non-enveloped viruses, and biofilms survive. This white paper demonstrates, based on evidence, the limits of area disinfection and how H₂O₂ cold fogging with CleanCube air + surfaces addresses in a validated 3D cycle – including clinical evidence (C. difficile infections −41%) and residue-free safety.
Applications in practice and industry
Steinemann Disinfection's 3 D disinfection technology is used in a wide variety of environments – wherever hygiene determines safety, efficiency and trust. Our systems enable reproducible disinfection processes that are validated, documented and residue-free. It treats both the air and any surface in the room – a crucial advance over manual procedures.
